Paula Ristow, Ana Bárbara Freitas Rodrigues, Leila Souza Fonseca, Waltee Martin Roland Oelemann, Guilherme Nunes Souza, Carla Dray Marassi, Eulógio Carlos Queiróz de Carvalho and Walter Lilenbaum


ABSTRACT


Paratuberculosis (PTB) is a chronic enteritis deter- mined by Mycobacterium avium subsp. paratuberculosis (Map) that affects ruminants. Three crossbred dairy cattle with clinical PTB were necropsied; tissue samples were collected and processed for histopathology and culture. Intestinal lesions were characterized in three levels of severity (mild, moderate and severe) by gross examination. Gross examination, microscopy and tissue culture were an- alyzed and compared. Mean tissue culture sensitivity was 66.6%. Map was recovered from 42.8% of intestinal mild lesions, 55.5% of intestinal moderate lesions, and 100% of severe intestine lesions. The results indicate a significant correlation between the level of lesions severity and the isolation of Map.


KEY WORDS: Cattle, Johne’s disease, Map, paratuberculosis, pathology.

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