Malaspina, Ana Carolina, Cavalcanti, Hebe Rodrigues, Leite, Clarice Queico Fujimaro, Machado, Silvia Maria Almeida, Viana, Brunilde Helena Jung, Silva, Rosangela Maria Gaspareto, Hage, Eduardo Ferraz, Figueiredo, Walter Machado, Marques, Elisabeth, Ferrazoli, Lucilaine, Arbex, Marcos, Lessi, Marcio, Fonseca, Leila S., Rigouts, Leen and Saad, Maria Helena Féres

Leonardo Silva Araujo, Renata Maciel Moraes, Anete Trajman, and Maria Helena Fe´res Saad1

Pleural tuberculosis (PL-TB) remains difficult to diagnose. An enzyme-linked immunosorbent assay (ELISA) was developed based on a construction containing the fusion of the Rv3019c (MT10.3) and Rv1980c (MPT64) gene sequences, and its performance was evaluated in an area where TB is endemic. A total of 92 pleural fluid (PF) samples at serial dilutions of 1:50 to 1:800 were included in the ELISA IgA MT10.3-MPT64 evaluation: 70 from TB patients and 22 from patients with other pleurisies. Confirmation of the expression and subsequent purification of the protein was made by SDS-PAGE and Western blot assays, resulting in a 36-kDa protein.

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A. Trajman, M. Pai, K. Dheda, R. van Zyl Smit, A.A. Zwerling, R. Joshi1, S. Kalantri, P. Daleye and D. Menzies

ABSTRACT:

Tuberculous pleuritis is a common manifestation of extrapulmonary tuberculosis and is the most common cause of pleural effusion in many countries. Conventional diagnostic tests, such as microscopic examination of the pleural fluid, biochemical tests, culture of pleural fluid, sputum or pleural tissue, and histopathological examination of pleural tissue, have known limitations. Due to these limitations, newer and more rapid diagnostic tests have been evaluated. In this review, the authors provide an overview of the performance of new diagnostic tests, including markers of specific and nonspecific immune response, nucleic acid amplification and detection, and predictive models based on combinations of markers. Directions for future development and evaluation of novel assays and biomarkers for pleural tuberculosis are also suggested.


KEYWORDS: Biomarkers, diagnosis, pleuritis, sensitivity, specificity, tuberculosis

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A Rede Brasileira de Pesquisa em Tuberculose (REDE-TB) é uma Organização Não Governamental (ONG) de direito privado sem fins lucrativos, preocupada em auxiliar no desenvolvimento não só de novos medicamentos, novas vacinas, novos testes diagnósticos e novas estratégias de controle de TB, mas também na validação dessas inovações tecnológicas, antes de sua comercialização no país e/ou de sua implementação nos Programa de Controle de TB no País.


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